Effect of oxidative stress on viability and virulence of environmental Acinetobacter haemolyticus isolates
نویسندگان
چکیده
The effect of oxidative stress exerted by 0.03% crystal violet, 0.3% bile salt, 4.0% NaCl and 8% ethanol on the survival and production of virulence factors among Acinetobacter haemolyticus isolates obtained from wastewater and river water samples was investigated. Though generally there was insignificant lethal effect against all the isolates, crystal violet exerted the highest lethal effect followed by ethanol and bile salt, NaCl exerted the least effects as compared to crystal violet and ethanol. Isolates from wastewater demonstrated the highest rate of resistance compared to isolates from river water. Presence of resistant verotoxic A. haemolyticus in the environments investigated is a cause for concern. Resistance exhibited by the bacteria means that the efficacy of these salts, chemicals and temperature conditions frequently employed as control/preservation agents in hospitals and food industries may be undermined.
منابع مشابه
Virulence, resistance genes, and transformation amongst environmental isolates of Escherichia coli and Acinetobacter spp.
The association of verotoxic E. coli and Acinetobacter spp. with various antibiotic-resistant, diarrhogenic, and nosocomial infections has been a cause for concern worldwide. E. coli and A. haemolyticus isolated on a number of selective media were screened for virulence factors, antibiotic resistance, and transformation of resistance genes. Out of 69 E. coli isolates obtained, 25 (35.23%), 14 (...
متن کاملTaxonomy of Acinetobacter: the usefulness of beta-D-xyloside xylohydrolase for strain differentiation.
One hundred and twenty-two clinical isolates of Acinetobacter were studied for the presence of beta-galactosidase and of beta-xylosidase, for biochemical characteristics, and for genetic interspecies transformation tests. All strains lacked beta-galactosidase; in contrast, beta-xylosidase was always present in the oxidative strains. This test proved to be of value for separating strains able to...
متن کاملFrequency of bap and cpaA virulence genes in drug resistant clinical isolates of Acinetobacter baumannii and their role in biofilm formation
Objective(s): Acinetobacter baumannii has a high propensity to form biofilm and frequently causes medical device-related infections with multiple-drug-resistance in hospitals. The aim of this work is to study antimicrobial resistance and the role of bap and cpaA genes in biofilm formation by A. baumannii to understand how this pathogen persists in the hospital environment. Materials and Methods...
متن کاملPrevalence Determination of Virulence Related and Biofilm Formation Genes in Acinetobacter baumannii Isolates from Clinical Respiratory Samples in Imam Khomeini Hospital, Tehran, Iran in 2018
Background and Objective: Acinetobacter baumannii is considered to be a re-emerging causative agent of nosocomial infections. There is a significant relation between pathogenicity of this bacterium and the numerous virulence factors. The purpose of this study was to investigate nine virulence factor genes in A. baumannii isolates derived from hospitalized patients. Materials and Methods: A tot...
متن کاملComparison of Distribution of Virulence Determinants in Clinical and Environmental Isolates of Vibrio cholera
Background: The virulence of a pathogenic Vibrio cholerae is dependent on a discrete set of genetic determinants. In this study, we determined the distribution of virulence determinants among the clinical and environmental isolates of V. cholerae. Methods: The antibiotic resistance profiles of the isolates were determined using standard disk diffusion assay. PCR assay was performed to analyze t...
متن کامل